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Identification and Genomic Characterization of Two Novel Porcine Circovirus Like Virus Strains Associated with Severe Diarrhoea and Hemorrhagic Enteritis in Piglets in China
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  • Xianhui Liu,
  • Xinming Zhang,
  • Hanqin Shen,
  • Kaiqi Lian,
  • Jihao Zheng,
  • Ge Xu,
  • Leyi Zhang,
  • Yanling Liu,
  • Linyang Yu,
  • Pengfei Zhang,
  • Jianguo Dong,
  • Changxu Song
Xianhui Liu
South China Agriculture University

Corresponding Author:[email protected]

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Xinming Zhang
South China Agriculture University
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Hanqin Shen
Wen’s Foodstuff Group Co. Ltd
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Kaiqi Lian
Anyang Institute of Technology
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Jihao Zheng
South China Agriculture University
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Ge Xu
South China Agriculture University
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Leyi Zhang
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Yanling Liu
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Linyang Yu
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Pengfei Zhang
South China Agriculture University
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Jianguo Dong
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Changxu Song
South China Agriculture UNiversity
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Abstract

The diarrhoea of pigs, in particular, for the newborns, is very harmful to the pig industry. Porcine circovirus-like virus (Po-Circo-like (PCL) virus) is a circular replication-associated protein (Rep)-encoding single-stranded (CRESS) DNA virus. Two PCL virus strains, with severe diarrhoea and hemorrhagic enteritis, have been found in two different pig farms in Guangdong province, China. Subsequently, the full genomes of two strains (PCL virus GD06 and PCL virus GD09) were sequenced. The two PCL viruses contains 3942 nucleotides and 3925 nucleotides, which vary from the genomes of other PCL virus strains with 3912, 3923, and 3942 nucleotides. Besides, the nucleotide identities between two strains and other strains of PCL viruses and Bo-Circo-like virus/CH were 78%-89%. A multiple sequence alignment of these strains showed a similarity of 86.2%-94.4% for the Rep gene sequence and 89.4%-97.7% for the Rep protein sequence. This study found that 9.5% (4/42) of diarrhoea samples and 11.8% (2/17) of pig farms were positive for PCL virus, suggesting that PCL virus may already be widespread in Pig farms in China. Further research on the pathogenicity and epidemiology of PCL virus is required.