Quarry operations cause serious environmental impact on invertebrate communities and contribute negatively to habitat destruction and the species they promote but very little is still known about the response of butterflies to such disturbances in western Africa. The current study provides data from a baseline survey of butterflies at Mowire quarry site in the agroecological zone of Ghana and investigates the effects of ongoing quarry operations on butterfly diversity. Specifically quarry operations were assessed on species abundance and richness. A total of 417 individual butterflies belonging to 67 species from 5 families was recorded in three zones. High relative abundance in the Eastern Zone (EZ) (N = 329) and high species richness (S = 55) may be due to increased flowering plants diversity which contain sweet nectar which attracts and support rich butterfly abundance. Low species richness (S = 22) and relative abundance (N= 41) in the Western Zone (WZ), and Northern Zone (NZ) (S = 21) and low relative abundance (N= 47), was corroborated with low plant diversity. Our results shows that, the EZ received less negative impact from the quarry operations but the operations negatively affected WZ and NZ. More generally our study indicates that state institutions should ensure mandatory environmental impact assessment reports from the quarry companies.