Piceatannol, a resveratrol analog, attenuates Dermatophagoides
farinae-induced atopic dermatitis like symptoms in NC/Nga mice
Abstract
Background: Piceatannol is a resveratrol metabolite commonly found in
red wine, grapes, and passion fruit seeds. Several studies have
investigated the immune-modulating effects of piceatannol on processes
related to allergic reactions. However, the relationship between
piceatannol and atopic dermatitis (AD) has not yet been reported.
Therefore, this study sought to investigate the effects of piceatannol
in animal and cell line models. Methods: AD-like symptoms and skin
lesions were induced by repeated topical application of Dermatophagoides
farinae extract (DFE) on the skin of NC/Nga mice. Piceatannol was
topically applied five times per week for four weeks. The molecular
mechanism of piceatannol was studied in the TNFα/IFNγ-induced HaCaT cell
line. Results: Topical application of piceatannol attenuated DFE-induced
AD-like symptoms, as shown by skin thickness, dermatitis score,
scratching time, and skin water loss. Histopathological analysis showed
that piceatannol suppressed DFE-induced eosinophil and mast cell
infiltration into the skin. These observations occurred concomitantly
with the downregulation of inflammatory markers, including serum TARC,
MDC, and IgE. In addition, piceatannol alleviated Th2 cytokines such as
IL-4 and IL-13 in the skin tissue. Piceatannol decreased phosphorylation
of JAK-STAT protein in the TNFα/IFNγ-induced HaCaT cell line. A
molecular docking study showed that piceatannol strongly interacts with
JAK1, suggesting a possible piceatannol mode of action. Conclusions:
Piceatannol, a metabolite of resveratrol, has potential therapeutic
efficacy in treating AD by targeting JAK1.