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Plastics degradation by hydrolytic enzymes: the Plastics-Active Enzymes Database - PAZy
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  • Patrick Buchholz,
  • Hongli Zhang,
  • Pablo Perez-Garcia,
  • Lena-Luisa Nover,
  • Jennifer Chow,
  • Wolfgang R Streit,
  • Juergen Pleiss
Patrick Buchholz
Universitaet Stuttgart

Corresponding Author:[email protected]

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Hongli Zhang
Universitaet Hamburg
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Pablo Perez-Garcia
Universitaet Hamburg
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Lena-Luisa Nover
Universitaet Hamburg
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Jennifer Chow
Universitaet Hamburg
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Wolfgang R Streit
Universitaet Hamburg
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Juergen Pleiss
Universitaet Stuttgart
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Abstract

Petroleum based plastics are durable and accumulate in all ecological niches. Knowledge on enzymatic degradation is sparse. Today, less than 50 verified plastics-active enzymes are known. First examples of enzymes acting on the polymers polyethylene terephthalate (PET) and polyurethane (PUR) have been reported together with a detailed biochemical and structural description. Further, very few polyamide (PA) oligomer active enzymes are known. In this paper, the current known enzymes acting on the synthetic polymers PET and PUR are briefly summarized, their published activity data were collected and integrated into a comprehensive open access database. The Plastics-Active Enzymes Database (PAZy) represents an inventory of known and experimentally verified plastics-active enzymes. Almost 3000 homologues of PET-active enzymes were identified by profile hidden Markov models. Over 2000 homologues of PUR-active enzymes were identified by BLAST. Based on multiple sequence alignments, conservation analysis identified the most conserved amino acids, and sequence motifs for PET- and PUR-active enzymes were derived.
01 Dec 2021Submitted to PROTEINS: Structure, Function, and Bioinformatics
01 Dec 2021Submission Checks Completed
01 Dec 2021Assigned to Editor
11 Dec 2021Reviewer(s) Assigned
29 Dec 2021Review(s) Completed, Editorial Evaluation Pending
03 Jan 2022Editorial Decision: Revise Major
28 Jan 20221st Revision Received
02 Feb 2022Submission Checks Completed
02 Feb 2022Assigned to Editor
02 Feb 2022Reviewer(s) Assigned
14 Feb 2022Review(s) Completed, Editorial Evaluation Pending
14 Feb 2022Editorial Decision: Accept
Jul 2022Published in Proteins: Structure, Function, and Bioinformatics volume 90 issue 7 on pages 1443-1456. 10.1002/prot.26325