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Microbial approaches for the assessment of toothpaste efficacy against oral species -- a method comparison
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  • Pune Paqué,
  • Lamprini Karygianni,
  • Julien Kneubühler,
  • Lorenzo Fiscalini,
  • Daniel B. Wiedemeier,
  • Marcel Müller,
  • Thomas Attin,
  • Thomas Thurnheer
Pune Paqué
University of Zurich

Corresponding Author:[email protected]

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Lamprini Karygianni
University of Zurich
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Julien Kneubühler
privat dental praxis in Zurich
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Lorenzo Fiscalini
privat dental praxis in Zurich
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Daniel B. Wiedemeier
University of Zurich
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Marcel Müller
University of Zurich
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Thomas Attin
University of Zurich
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Thomas Thurnheer
University of Zurich
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Antibacterial properties of toothpastes enable chemical plaque control in limited-access tooth regions that are mechanically not sufficiently reached by toothbrushes. Therefore, this study aimed to compare different microbial methods to assess antimicrobial toothpaste properties and evaluate different toothpastes in terms of their antibacterial efficacy against different oral microorganisms in vitro. Six toothpaste suspensions with varying antibacterial supplements were applied to a multi-species biofilm model (Actinomyces oris, Candida albicans, Fusobacterium nucleatum, Streptococcus oralis, Streptococcus mutans) as well as to each microorganism. A culture method was used to assess the anti-biofilm effects and two different agar diffusion assays were performed for testing the antimicrobial effect on each microorganism. The measurements of the culture and diffusion analyses were statistically normalized and compared and toothpastes were ranked according to their antimicrobial efficacy. The results of both agar diffusion assays showed a high correlation across all tested species (Spearman correlation coefficients ρs > 0.95). The results of the multi-species biofilm model, however, substantially differed in its assessment of antibacterial properties (ρs ranging from 0.22 to 0.87), compared to the results of both diffusion assays. Toothpastes with amine fluoride with and without stannous fluoride, and triclosan resulted in the highest antimicrobial efficacy, while activated carbon supplements were comparable to the negative control NaCl. The appropriate selection of a broad range of oral microorganisms seems crucial when testing the chemical impact of toothpastes and toothpaste supplements.
30 Aug 2021Submitted to MicrobiologyOpen
08 Sep 2021Submission Checks Completed
08 Sep 2021Assigned to Editor
08 Sep 2021Review(s) Completed, Editorial Evaluation Pending
08 Sep 2021Editorial Decision: Revise Minor
21 Sep 2021Reviewer(s) Assigned
20 Sep 20211st Revision Received
11 Jan 20222nd Revision Received
11 Jan 2022Assigned to Editor
11 Jan 2022Submission Checks Completed
11 Jan 2022Review(s) Completed, Editorial Evaluation Pending
11 Jan 2022Reviewer(s) Assigned
02 Feb 2022Editorial Decision: Revise Minor
15 Feb 20223rd Revision Received
16 Feb 2022Submission Checks Completed
16 Feb 2022Assigned to Editor
16 Feb 2022Review(s) Completed, Editorial Evaluation Pending
16 Feb 2022Editorial Decision: Accept