loading page

To split or not to split: CASP15 targets and their processing into tertiary structure evaluation units
  • Andriy Kryshtafovych,
  • Daniel Rigden
Andriy Kryshtafovych
University of California Davis Genome Center

Corresponding Author:[email protected]

Author Profile
Daniel Rigden
University of Liverpool Institute of Systems Molecular and Integrative Biology
Author Profile


Processing of CASP15 targets into evaluation units (EUs) and assigning them to evolutionary-based prediction classes is presented in this study. The targets were first split into structural domains based on compactness and similarity to other proteins. Models were then evaluated against these domains and their combinations. The domains were joined into larger EUs if predictors’ performance on the combined units was similar to that on individual domains. Alternatively, if most predictors performed better on the individual domains, then they were retained as EUs. As a result, 112 evaluation units were created from 77 tertiary structure prediction targets. The EUs were assigned to four prediction classes roughly corresponding to target difficulty categories in previous CASPs: TBM (template-based modeling, easy or hard), FM (free modeling), and the TBM/FM overlap category. More than a third of CASP15 EUs were attributed to the historically most challenging FM class, where homology or structural analogy to proteins of known fold cannot be detected.
13 Mar 2023Submitted to PROTEINS: Structure, Function, and Bioinformatics
13 Mar 2023Submission Checks Completed
13 Mar 2023Assigned to Editor
13 Mar 2023Review(s) Completed, Editorial Evaluation Pending
25 Mar 2023Reviewer(s) Assigned
10 Apr 2023Editorial Decision: Revise Minor
02 May 20231st Revision Received
03 May 2023Submission Checks Completed
03 May 2023Assigned to Editor
03 May 2023Review(s) Completed, Editorial Evaluation Pending
03 May 2023Reviewer(s) Assigned
18 May 2023Editorial Decision: Accept