Mitochondrial transcription is being studied with the increasing interest to better understand the coding capacity of mitochondrial DNA (mtDNA). Based on studies showing that mtDNA encodes additional genes that are not functional in oxidative phosphorylation (OXPHOS), it has been understood that mitochondrial transcription is more complex than previously thought. In this study, mitochondrial transcription was investigated in terms of polyadenylation patterns in Apis mellifera (Insecta: Hymenoptera). We found that both sense and antisense transcripts were captured and polyadenylated, and gene expression levels and polyadenylation length were highly variable between genes in Apis. Mitochondrial RNAs of A. mellifera were found to be polyadenylated with an average size of 31 adenines. Nevertheless, the highest transcript representatives of each gene generally appeared to have polyadenylation of size 5-9 bases. Generally, transcripts containing longer and/or shorter forms of each gene were thought to indicate the existence of putative UTR-containing genes, functional non-coding RNA genes and variant gene forms. We showed that some transcripts contain non-template dependent additional nucleotides before polyadenyl tail. Transcripts containing at least some bases of the intergenic regions downstream of the gene had the highest transcript representation after the monocistronic gene units. These findings support the possible functional role of the intergenic regions.