Fig 9: The concentration- dependent mortalities of Biomphalaria juveniles exposed to ethanol extracts of pumpkin seeds.
The LC50 values extrapolated from the non-linear regression line from Graph pad Prism were way below the maximum LC50 value of 40mg/L set by the WHO. The LC50 value of water and ethanol extracts of pumpkin seeds on adult Biomphalaria had the lowest LC values of 0.002mg/ml. The water extracts LC values on juvenile Biomphalaria snails and adult Bulinus snails were both 0.004mg/ml. The LC50 for ethanol extracts on juvenile Biomphalaria snails was 0.19mg/ml. These results are presented n Table 1.
Table 1 showing LC50 values of pumpkin seed extract on the five snail classes.