• Does the title accurately reflect the results?
  • Experimental design: were the techniques appropriate for the hypothesis? Were suitable controls included? Were new techniques/mutants/cell lines etc. sufficiently characterised to allow the study to be replicated? Were robust statistical methods used?
  • Do the results support the authors’ conclusions and, if not, what additional experiments would you suggest?
  • Are there big flaws in the understanding of the current literature and interpretation of the literature that might undermine the interpretation of the current results?