Conclusion
In summary, we present the most complete H. glycines assembly,
with a consensus gene prediction pipeline sensitive to the prediction of
high-copy parasitism-related genes. We confirm this with a high
percentage of synteny to previous assemblies, high read mapping rates,
and the complete integration of all contigs into nine pseudomolecules.
Using currently available data, we compiled a comprehensive resource
that extensively annotates H. glycines genes, a critical resource
for the development of advanced technology to combat this pest. This
resource will be integrated into SCNBase.org, which further extends the
transparency and availability of H. glycines genomic data. To
demonstrate the utility of this new resource, we assessed the
conservation of previously published effectors and leveraged published
RNAseq and gene features to further explore effector expression during
the host-parasite exchange.
Data availability
All scripts and notes used to prepare this genome are available at
Dovetail2SCNGenome@github.com.
The genome, annotation, and Hi-C reads were uploaded to Genbank and SRA
under the Bioproject PRJNA603076 and SRR8381095. All genome track data
and annotations will also be hosted on SCNBase.org.
Author Contributions
Conceptualization – REM, AJS, TM, TB; Data curation – REM, MH; formal
analysis – REM, AS, TM; funding acquisition – AJS, TM, TB;
investigations – REM, AJS, TM, TB; methodology – REM, TM, AJS, TB;
resources – AJS, TM, MH, TB; Software – REM, AJS; Validation – REM;
Visualization – REM; Writing – REM; Review and Editing – REM, TM, MH,
AJS, TB