Fig. 1. Overview of rational metabolic engineering procedure.
Following introduction of 13C-labeled sodium
propionate, a series of metabolite samples were obtained by rapid
quenching and extraction. Dynamic changes in isotope labeling patterns
were assessed using LC-MS/MS, followed by strain construction and
computational analysis of these trajectories to estimate metabolic
pathway fluxes.
Fig. 2. Schematic representation of single-point fast sampling
device