COL13 RNA accumulates at high levels in hypocotyls
By searching the gene expression information in the Arabidopsis Information Resource (TAIR) database (Klepikova, Kasianov, Gerasimov, Logacheva, & Penin, 2016), we found that COL13(AT2G47890 ) is highly expressed in the hypocotyl. qRT-PCR analysis confirmed thatCOL13 was expressed in most plant organs, with higher expression in the hypocotyl and stem (Fig. 1a). To determine the spatial patterns of COL13 expression in more detail, transgenic lines expressing GUS driven by the 2812 bp COL13 promoter fragment were generated. As shown in Fig. 1b, GUS expression was predominantly in the hypocotyl.