Figure 2. Effect of IFN-γ treatment on peripheral blood monocyte
HLA-DR expression. (A) Whole blood was treated with 100 ng/mL of LPS,
100 ng/mL of IFN-γ or both. Surface monocyte HLA-DR expression assessed
by FACS at 0 h, 6 h and 18 h. Values are mean ± S.E.M. N = 6. * P
< 0.05, Repeat Measures ANOVA with post-hoc Holms-Sidak test.
(B) Representative flow cytometry images with the control group shown in
grey and each experimental condition shown without shading. A minimum of
20,000 events acquired for each comparison. MFI, mean fluorescence
intensity; LPS, lipopolysaccharide; IFN-γ, interferon-gamma.