Cytokine analysis
Stored plasma was used to determine cytokine concentrations using Enzyme-linked immunosorbent assays (ELISAs) according to the manufacturer’s protocol (eBioscience, San Diego, CA). Samples were measured in duplicate and compared against a known standard curve. The lower limits of detection were 2 pg/mL and 4 pg/mL for IL-10 and TNF-α, respectively.