HLA-DR expression
In control conditions, monocyte HLA-DR expression remained low throughout the time points measured (Fig. 2). LPS increased monocyte HLA-DR expression to 1190.27 (± 178.73) MFI at 6 h (p = < 0.001) and this remained similarly elevated at 18 h when compared with control (p = 0.006).
IFN-γ treatment alone did not affect HLA-DR expression at 6 h, and by 18 h expression was higher than control conditions but still only 521.89 (± 76.01) MFI (p = 0.243).
Treatment with IFN-γ with LPS demonstrated similarly elevated monocyte HLA-DR expression at 6 h as LPS treatment alone (p = 0.843). However, at 18 h monocyte HLA-DR expression was significantly higher than with LPS without IFN-γ (p = 0.018) (Fig. 2).