Figure 4. Effect of IFN-γ treatment on peripheral blood T-cell function. (A) Whole blood was treated with 100 ng/mL of LPS, 100 ng/mL of IFN-γ or both. After 16 h of experimental conditions, PBMC’s were isolated, washed then stimulated with PMA, ionomycin and Brefeldin A for 6 h. Intracellular IFN-γ production was measured in CD4 and CD8 T-cells by FACS as a measure of T-cell activation. Values are mean ± S.E.M. N = 6 for each condition. *P < 0.05, Repeat Measures ANOVA with post-hoc Holms-Sidak test. (B) Representative flow cytometry images with the control group shown in grey and each experimental condition shown without shading. A minimum of 20,000 events acquired for each comparison. MFI, mean fluorescence intensity; LPS, lipopolysaccharide; IFN-γ, interferon-gamma.