Figure 4. Effect of
IFN-γ treatment on peripheral blood T-cell function. (A) Whole blood
was treated with 100 ng/mL of LPS, 100 ng/mL of IFN-γ or both. After 16
h of experimental conditions, PBMC’s were isolated, washed then
stimulated with PMA, ionomycin and Brefeldin A for 6 h. Intracellular
IFN-γ production was measured in CD4 and CD8 T-cells by FACS as a
measure of T-cell activation. Values are mean ± S.E.M. N = 6 for each
condition. *P < 0.05, Repeat Measures ANOVA with post-hoc
Holms-Sidak test. (B) Representative flow cytometry images with the
control group shown in grey and each experimental condition shown
without shading. A minimum of 20,000 events acquired for each
comparison. MFI, mean fluorescence intensity; LPS,
lipopolysaccharide; IFN-γ, interferon-gamma.