GC-MS analyses
GC analysis was conducted using a Varian 3400 apparatus (Palo Alto, CA,
USA) equipped with a flame ionization detector and a Stabilwax‐DA
capillary column (RESTEK, Bellefonte, US; Dimension:
15 m × 0.32 mm × 0.25 μm). The oven temperature program was set to
140–230 °C, with 5 °C min−1 increments, with a 2 and
10 min delay at the initial and final temperatures, respectively. Total
run time was 30 min and carrier gas (nitrogen) pressure was
approximately 70 kPA. The linseed oil samples
were trans esterified to form fatty acids methyl esters (FAMEs)
by alkaline‐catalysis using AOCS official method Ce 2‐66 (AOCS 1997).
For the analyses, 20 μL of the analyzed samples were dissolved in 700 μL
heptane, and 1 μL of the supernatant was injected to the GC.
Identification of peaks in the chromatogram was performed using a
rapeseed FAME’s mix standard purchased from Sigma–Aldrich.
Gas chromatography-mass spectrometry analyses (GC-MS) was performed
using an GC-MS system SCION™ (Bruker, USA), GC-436. Detection was
carried out with SQ mass-selective single quadrupole detector. The GC-MS
operation control and data process were carried out by Bruker MS
Workstation 8 SP2 for SCION™ software package.
The Varian GC capillary column part number CP7419, J&W Select FAME GC
Column, L (m) x ID (mm ) x OD(mm): 50 x 0.25 X0.39, format 7 inch,
capillary tubing: fused silica, mid polarity, temperature range: 45°C-
275/290°C (made in the Netherlands) was used.
The injector temperature was 280◦C.The oven temperature was held at 70◦C
for 1 min, then increased to 270◦C at heating rate 7◦C/ min. The carried
gas was helium (purity 99.999%) at flow rate 1.6 ml/ min. The sample
volume was 1 µl. The conditions for election impact ionization (EI) were
an ion energy of 70 eV and mass range scanning was 39-500 m/z.