Effect of anti-properdin on alternative pathway activation in serum
IgG from LN patients, positive for anti-properdin autoantibodies and healthy donors were added to normal human serum and incubated 1h at 37°C to test their capacity to activate complement in fluid phase. The released Ba fragment was measured as an indicator for the formation of a C3 convertase. The test was performed according manufacturer’s procedure, MicroVue Ba kit (Quidel).