Meso Scale Discovery (MSD) multiplex analysis
Cytokine levels in the microglia cell culture media were measured with the Meso Scale Discovery (MSD, USA) proinflammatory mouse V-Plex Plus kit (22).
Statistics
The statistical analyses were performed in Prism 6.01 (GraphPad) using one-way ANOVA followed by Sidak’s or Dunnett’s multiple comparisons tests or by unpaired T test with Welch’s correction. Outliers were removed using the ROUT method (Q=1%). Differences among means with p<0.05 were accepted as statistically significant, while differences among means with 0.05<p<0.10 were accepted as representing tendencies of difference.