The effect of cladribine on the mRNA expression of DCK in LPS- and IL-4-stimulated microglia
mRNA expression of deoxycytidine kinase (DCK) was quantified by qPCR and is shown relative to DMSO control. A. Microglia were treated with DMSO (28.2 mM), LPS (10 ng/ml), DMSO and LPS, and different concentrations of CdA (0.01-10 µM) together with LPS for 24 hours.B. Microglia were treated with DMSO (28.2 mM), IL-4 (20 ng/ml), DMSO and IL-4, and different concentrations of CdA (0.01-10 µM) together with IL-4 for 24 hours.
**p≤0.01, ***p≤ 0.001, n=3-4 in each group, one-way ANOVA followed by multiple comparisons tests, mean±SD.