The effect of cladribine on the phagocytotic ability and random
migration of microglia
A. Phagocytosis was quantified after microglia were treated
with DMSO (28.2 mM), LPS (10 ng/ml), DMSO and LPS, different
concentrations of CdA (0.01-10 µM), and different concentrations of CdA
together with LPS for 24 hours. Fluorescent latex beads were added to
the cell media for 40 minutes. As negative control, microglia were
treated with cytochalasin D (5 µg/ml) for 30 minutes before addition of
latex beads. The cells were analyzed by flow cytometry, and the
phagocytotic ability was quantified by mean fluorescent intensity (MFI)
and presented relative to DMSO control. B. Track displacement
was quantified by analysis of timelapse videos using TrackMate after
microglia were treated with DMSO (28.2 mM), LPS (10 ng/ml), DMSO and
LPS, different concentrations of CdA (0.1-10 µM), and different
concentrations of CdA together with LPS for 24 hours. The results are
shown relative to DMSO control. C. Total distance travelled was
quantified by analysis of timelapse videos using TrackMate after
microglia were treated with DMSO (28.2 mM), LPS (10 ng/ml), DMSO and
LPS, different concentrations of CdA (0.1-10 µM), and different
concentrations of CdA together with LPS for 24 hours. The results are
shown relative to DMSO control.
*p≤0.05, ****p≤0.0001, n=3-5 in each group, one-way ANOVA followed by
multiple comparisons tests, mean±SD.