RNA extraction and quantitative PCR
RNA was extracted using RNeasy micro kit (Qiagen, Denmark) and reverse transcribed using High Capacity cDNA Reverse Transcription kit (Applied Biosystems, 4374966). Quantitative PCR (qPCR) was performed on Bio-Rad CFX ConnectTM Real-Time System (Software Bio-Rad CFX Manager 3.1) using SYBR green chemistry (Thermo Scientific) and corresponding primers (Supplementary Table 1 ). The expression levels are reported relative to the geometric mean of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and hypoxanthine phosphoribosyltransferase 1 (HPRT1).