Figure 2
The effect of cladribine on microglia granularity and size
Microglia were treated with DMSO (28.2 mM), LPS (10 ng/ml), DMSO and LPS, different concentrations of CdA (0.01-10 µM), and different concentrations of CdA together with LPS for 24 hours. A. A representative example of size (FSC) and granularity (SSC) of microglia by flow cytometry. B. The effect of CdA on the granularity (SSC) of naïve and LPS-stimulated microglia relative to DMSO control. C. The effect of CdA on the size (FSC) of naïve and LPS-stimulated microglia relative to DMSO control.
***p≤0.001, n=3-4 in each group, one-way ANOVA followed by multiple comparisons tests, mean±SD.