The effect of cladribine on the mRNA expression and protein
secretion of cytokines by LPS-stimulated microglia
A. mRNA expression of IL-1β, TNF and IL-10 was quantified by
qPCR after microglia were treated with DMSO (28.2 mM), LPS (10 ng/ml),
DMSO and LPS, and different concentrations of CdA (0.01-10 µM) together
with LPS for 24 hours. The expression is shown relative to DMSO control.B. The protein concentration of IL-1β, TNF and IL-10 in the
microglia cell culture supernatant was quantified by Meso Scale. The
microglia were treated with DMSO (28.2 mM), DMSO and LPS, and different
concentrations of CdA (0.1-10 µM) together with LPS for 24 hours.
*p≤0.05, **p≤0.01, ***p≤ 0.001, ****p≤0.0001, n=4 in each group,
unpaired t test with Welch’s correction and one-way ANOVA followed by
Dunnett’s multiple comparisons test, mean±SD.