Cladribine (0.1-1 µM) decreases the phagocytotic ability of
LPS-stimulated microglia
To investigate the effect of CdA on phagocytosis by naïve and
LPS-stimulated microglia, the cells were incubated with fluorescent
latex beads, and analyzed by flow cytometry. LPS stimulation
significantly increased phagocytosis compared to control, while
treatment with cytochalasin D, an inhibitor of actin polymerization,
reduced phagocytosis (Figure 3A ). CdA in the range of 0.1-1 µM
significantly decreased phagocytosis by LPS-stimulated microglia, while
treatment with CdA (0.01-10 µM) did not affect phagocytosis by naïve
microglia compared to DMSO control (Figure 3A ).