Botrytis cinerea cultivation, infection and scoring
B. cinerea R16 (Faretra and Pollastro, 1991) was used in all experiments and was kindly provided by Dr Mike Roberts (Lancaster University). Cultivation of the fungus and infection of tomato-based experiments were performed as described (Luna et al., 2016). ForN. benthamiana, 2-3 detached leaves were inoculated with 6µl inoculum solution containing 2 x 104 spores/ml ofB. cinerea . Infected leaves were kept at 100% RH by sealing the trays and placed in the dark before disease assessment. Arabidopsis infections were performed as previously described (La Camera et al., 2011) with a few modifications. Leaves were inoculated with 5µl inoculum solution containing ½ strength of potato dextrose broth (PDB – Difco at 12 g/l) and 5 x 105 spores/ml. Infected Arabidopsis plants were put in a sealed tray at 100% RH and moved back to the growth cabinet. Infections of S. melongena plants were performed by drop inoculating detached leaves with a spore solution of B. cinerea containing 2 x 104 spores/ ml. For all pathosystems, disease was scored by measuring lesion diameters with an electronic calliper (0.1 mm resolution) on different days post infection.