Morpholinos and RNA injections
The effect of zebrafish patched1 and patched2 morphants
has been previous reported (Koudijs et al., 2008) and, therefore, we
ordered Morpholino Oligonucleotides (MO) targeting each translation
start site of zebrafish patched1 and patched2 mRNA, as
well as control MOs, were developed by Gene Tools (Gene Tools LLC) and
designed as follows: the patched1 translation-blocking MO (ATG
MO), 5’-AGGAGACATTAACAGCCGAGGCCAT-3’ and the patched2 ATG MO,
5’-CATAGTCCAAACGGGAGGCAGAAGA-3’. MOs were diluted in RNAse-free water to
0.1-0.5 ng/nl (ptch 1, ptch 2), 5 ng/nl (con MO).