Suppl. Figure 2
The results of SMO mRNA dose-response pilot experiments for our
over-expression bioassay development. Abnormal phenotypes are not
consistently observed with single injections of low dose of SHHor even a high dose of SMO . On the other hand, a 2pg ofSHH and 200pg of SMO mRNA (co-injection) significantly
induced abnormal phenotypes compared with all other dose conditions
[except 1pg of SHH and 600pg of SMO ]. For this pilot
study, the injections for 50 embryos on each group were performed in
triplicate and on different days. Error bars represent means with SD.
**P < 0.01 (Student’s t-test).