Figure 4.
Functional analysis of representative domain variants were conducted
using low dose MO of ptch1 and ptch2 . WT zebrafish embryos
were injected with low dose ptch1 and ptch2 MO as well as
with each of the four distinct SMO mRNA transcripts and harvested
at 24hpf. (A ) The eye area was measured at 24hpf. Significant
differences in eye area observed in SMO p.V404M when compared togfp , SMO p.R113Q, p.R199W and p.P687L. Representative eye
images of gfp (a) and SMO p.V404M (b) are shown upper
panels. (B ) Somite angle was measured at 24hpf. Significant
difference in somite angle observed in SMO p.R113Q when compared
to gfp , SMO p.R199W and p.P687L. Representative somite
images of gfp (a’) and SMO p.R113Q (b’) are shown upper
panels. Error bars represent means with SD. *P < 0.05
(Student’s t-test).