Position in IsPETase
Amino acid or gap symbol
Annotations or substitutions
References
87 Y 51%, F 46% Subsite I, aromatic clamp, oxyanion hole 38,39
88 T 76% (V 7%, L 5%, M 1%) Subsite II 39
Thermostability: T96M in LCC 40
89 A 70% (G 12%, S 10%) Subsite II 39
119 Q 69% (F8%, Y4%, W2%) Subsite I 39
Thermostability: Y127G in LCC 40
139 Gap 91% Extension in α-helix 2 38
140 Gap 80% Extension in α-helix 2 38
141 Gap 90% Extension in α-helix 2 38
159 H 87% (W9%) Subsite II 38,39
160 S 100% Catalytic triad 38
161 M 94% Subsite I, oxyanion hole 39
185 W 77% (Y12%) Subsite I, aromatic clamp, ”wobbly tryptophane” 38,39
206 D 100% Catalytic triad 38
208 V 54% (I 36%) Substrate interaction 38
237 H 100% Catalytic triad 38
238 F 64% (L 11%, S 8%, Y 3%) Subsite II 38,39
Binding & activity: F243I in LCC 40
Binding & activity: F243W in LCC 40
241 N 56% (Q 12%) Subsite II 39
Thermostability: N246D in LCC 40
Thermostability: N246M in LCC 40
242 (S 36%, T 32%, G 5%, I 4%) Extended loop 38,39
243 (S 40, G 11%, T 8%) Extended loop 39
244 N 74% (G 3%, Y 3%) Extended loop 39
245 - 87% Extended loop 39
246 - 89% Extended loop 39
247 - 87% Extended loop 39