3.5 Phylogeny and divergent time
Using the genomes and genes of 10 selected teleosts, we identified a total of 4,134 were single copy orthologues, based on which a phylogenetic tree was constructed, revealing the phylogenetic relationships of the selected teleost (Figure 4 ). We found that the P. leopardus diverged ~ 57.2 million years ago (mya) from the common ancestor with the linage ofE. lanceolatus and E. akaara . The most closely related species to the grouper linage is G. aculeatus , which separated with their common ancestor ~71.2 mya (Figure4 ).