Figure 1. A photograph of a red P. leopardus (by Qian
Zhou).
Figure 2. Genome size estimation and assembly. (a) Graph ofk -mer frequency distribution (k = 17) generated from
127.36 Gb sequencing data of P. leopardus . The highest peak at
coverage 66× corresponds to the homozygous peak. The minor peak at
coverage 35× corresponds to the heterozygous peak. The minor peak at
coverage 138× corresponds to duplications. The illustrations of the
lines are marked in the graph. (b) The Hi-C contact map of the P.
leopardus genome. The color bar shows the contact density from white
(low) to red (high).
Figure 3. Comparisons of the predicted gene models betweenP. leopardus genome and other teleost species. (a) mRNA length.
(b) CDS length. (c) Exon length. (d) Intron length. (e) Exon number.
Figure 4. Phylogenetic tree constructed using 4134
single copy orthologues among the selected teleost, with 500
bootstraps. The estimated divergent time (Mya, million years ago) and
the 95% confidential intervals were labeled at each branch. The red
dots indicate the divergent time used for re-calibrations.
Figure 5. Comparative genomic analyses of P.
leopardus with two grouper species, E. lanceolatusand E. akaara) . (a) Chromosomal collinearity betweenP. leopardus and E. lanceolatus. The colorful bars
(Chr1-24) and the green bars (I-IIXIV) indicate each of the 24
chromosomes in P. leopardus and E. lanceolatus ,
respectively. (b) Venn diagram of the genes from the three grouper
species. (c) Gene family expansion and contraction. (d) The enrichment
of KEGG annotations with the expanded gene families in P.
leopardus (p < 0.05).