rs103294 and rs410852 genotyping
The genotypes of rs103294 and rs410852 were detected by the PCR-ligation
detection reaction (PCR-LDR) method with technical support from Shanghai
Biowing Applied Biotechnology Company (Shanghai,
China)[28,29]. The sequences of the primers and
probes are listed in Supplementary Table 2. The PCR product was used as
the template in the LDR reaction, and the LDR products were assessed
using the ABI 3730 sequencer (ABI, Carlsbad, USA).