Cell culture
The human U937 monocyte cell line (Cat. FDCC-HLC063) and 293T cells
(Cat. FDCC-HSN190) were obtained from Fudan Cell Center (F.D.C.C) of
Fudan University (Shanghai, China). Identification of the two cell lines
were at the China Centre for Type Culture Collection in Wuhan, China.
The U937 cells were cultured with RPMI-1640 medium (HyClone, Logan,
USA); 293T cells were cultured in DMEM medium (HyClone, Logan, USA)
containing 10%fetal bovine serum (FBS) (HyClone, Logan, USA),
penicillin (100 U/ml) and streptomycin (100 µg/ml) in humidified 5%
CO2 at 37°C.