Elisa
Culture media of U937 cells were collected and centrifuged at 20,000 × g for 1 min. The supernatants were aliquoted and stored at ˗80°C until analysis. The concentration of IFN-gamma, IL-6, TNF-α, CCL2, CCL3, CXCL8/IL-8 and CXCL10 was measured using ELISA kits (NeoBiosciences, Shenzhen, China), according to the manufacturers’ protocol.