Phylogenetic trees of different hosts and genotypes were constructed based on the complete Bo/CH/HB/BD/2019 genome sequence and the complete genomes in the GenBank database (Figure 1). Figure 1a shows that bovine isolates and human isolates are far related, so there is no potential risk of cross-infection at present. However, BNoV (GIII) serum antibodies have been detected in humans (Widdowson et al., 2005) and human NoV sequences have been detected in bovine fecal samples (Menon et al., 2013), and interspecific transmission may occur in the future. Figure 1b shows that all BNoV isolates belonged to the GIII genotype. The Bo/CH/HB/BD/2019 strain is more closely related to the other two Chinese strains than to those from other countries, and is hence clustered in the Chinese BNoV branch in the phylogenetic tree. The isolated strain was more closely related to the Bo/HN-1/2018/China strain from Henan (central China) (GenBank accession number: MN122335.1) than to the Bo/BET-17/18/CH strain from Sichuan (southern China) (GenBank accession number: MK159169.1). This suggests that there may be a different evolutionary process underlying the spread of BNoV in China. Unfortunately, few data are available on the genetic evolution and prevalence of BNoV in China.