Multiple alignment of the surface glycoprotein and
identification of sequence variants
Surface glycoproteins sequences obtained from the GenBank were aligned
using the multiple sequence alignment feature of the BLAST sequence
analysis tool from NCBI (Supplementary Figure 1) (Altschul et al.,
1997). The first sequence SARS-CoV-2 from isolate Wuhan-Hu-1 (Accession:
MN908947.3; Wuhan, China) was used as the consensus sequence to
determine subsequent variant sequences from the different isolates (Wu
et al., 2020).
Furthermore, the surface glycoprotein sequences obtained from GISAID
were analyzed for similarity by using the multiple sequence alignment
tool Clustal Omega (Supplementary Figure 2) (Madeira et al., 2019).
Again, the sequences were compared to the Wuhan-Hu-1 isolate (Accession:
MN908947.3) to determine divergent sequences. Also, multiple sequence
alignment for all isolates from Belgium was conducted separately
(Supplementary Figure 4). The multiple sequence alignments were analyzed
using the software BioEdit to identify the sequence differences between
isolates.