Fig. S6. Costs of Pc-elicited TAR in individual F1 populations and mismatched environments. See legends of Fig. 6c,d for details. (a) Hpa resistance in F1 plants from Pc-exposed parents. Stacked bars show leaf frequency distributions across Hpa resistance classes within F1 populations from similarly treated parents (n=80-130). P-value indicates statistical significance of parental treatment (Fisher’s exact test). Different letters indicate statistically significant differences between pooled F1 populations from similarly treated parents (Pairwise Fisher’s exact tests + Bonferroni FDR; α=0.05). (b) Root growth of F1 plants from mock- and Pc-treated parents at 0, 50 and 100 mM NaCl. Boxplots show the interquartile range (IQR; box) ± 1.5xIQR (whiskers), including median (horizonal line) and replication units (dots). Data represent root growth values (cm) of single plants within individual F1 populations over a 5-day period (n=10). P-values indicate statistical significance of parent treatment, F1 treatment and interaction by 2-way ANOVA of pooled F1 populations from similarly treated parents, and nested 2-way ANOVA with F1 population as random factor, respectively. NS: no statistically significant differences between pooled F1 populations from similarly treated parents (ANOVA + Tukey post-hoc test; α=0.05). (c) Tolerance of F1 plants from mock- and Pc-treated parents to 50 and 100 mM NaCl. Tolerance was quantified by root growth reduction (%) relative to the mean root growth value at 0 mM NaCl of the corresponding F1 population (Fig. S6b). Boxplots show the interquartile range (IQR; box) ± 1.5xIQR (whiskers), including median (horizonal line) and replication units (dots). Data represent root growth reduction percentages of single plants within individual F1 populations. P-values indicate statistical significance of parent treatment, F1 treatment and interaction by 2-way ANOVA of pooled F1 populations from similarly treated parents, and nested 2-way ANOVA with F1 population as random factor, respectively. NS: no statistically significant differences between pooled F1 populations from similarly treated parents (ANOVA + Tukey post-hoc test; α=0.05).