Fig. S5. Costs of Pst-elicited TAR in individual F1 populations and mismatched environments. All boxplots show the interquartile range (IQR; box) ± 1.5xIQR (whiskers), including median (horizonal line) and replication units (dots). See legends to Fig. 6a,b for details. (a) Pc resistance of F1 plants from mock- and Pst-treated parents 6 dpi. Data represent lesion diameters (mm) of plants within individual F1 populations (n=38-40). P-values indicate statistical significance of parent treatment by ANOVA of pooled F1 populations from similarly treated parents, and nested ANOVA with individual F1 population as random factor, respectively. Different letters indicate statistically significant differences between pooled F1 populations from similarly treated parents (ANOVA + Tukey post-hoc test; α=0.05). (b) Root growth of F1 plants from mock- and Pst-treated parents at 0, 50 and 100 mM NaCl. Data represent root growth values (cm) of plants within individual F1 populations over a 5-day period (n=10). P-values indicate statistical significance of parent treatment, F1 treatment and interaction by 2-way ANOVA of pooled F1 populations from similarly treated parental plants, and nested 2-way ANOVA with F1 population as random factor, respectively. For each NaCl concentration, different letters indicate statistically significant differences between pooled F1 populations from similarly treated parents (ANOVA + Tukey post-hoc test; α=0.05; NS: no statistically significant differences). (c) Tolerance of F1 plants from mock- and Pst-treated parents to 50 and 100 mM NaCl. Tolerance was quantified by root growth reduction (%) relative to the mean root growth value at 0 mM NaCl of the corresponding F1 population (Fig. S5b). Data represent root growth reduction percentages of single plants within individual F1 populations (n=10). P-values indicate statistical significance of parent treatment, F1 treatment and interaction by 2-way ANOVA of pooled F1 populations from similarly treated parents, and by nested 2-way ANOVA with F1 population as random factor, respectively. For each NaCl concentration, different letters indicate statistically significant differences between pooled F1 populations from similarly treated parents (ANOVA + Tukey post-hoc test; α=0.05; NS: no significant differences).