Bacteriological examination and identification
Phenotypically, all Brucella isolates (n=41) proved to beB. melitensis bv3 by fitting the identification scheme of Alton
et al. (1988) and OIE, (2018). AMOS-PCR showed B.
melitensis specific band of 731 bp. Thirty-one isolates were obtained
from 28 sheep and 3 goats (the preferential host), and ten isolates were
obtained from nine cattle and one
buffalo (non-preferential hosts) as, shown in Table 2.