Sequence Analysis
PCR products from DNA and cDNA were purified using a Qiaquick PCR
purification Kit (Qiagen TM, ME, DE) and bidirectionally sequenced using
a BigDye Terminator v1.1 Cycle Sequencing Kit (Applied Biosystems, CA,
USA) following the manufacturer’s recommendations. Sequences were
removed with a DyeEx_2.0 spin kit (Qiagen TM, DE) and run on a
SeqStudio Genetic Analyzer (Thermo Fischer Scientific, CA, USA).
Electropherograms were analyzed using Sequencing analysis v5.2 and
sequence scanner v1.0 software (Thermo Fischer Scientific, CA, USA). The
obtained sequences were compared to other sequences in GenBank using
BLAST.