Antibodies and reagents-REDO
The anti-human antibodies and isotype controls that were used included: directly conjugated CD25 FITC, Foxp3 Alexa647, CD183 PE (CXCR3), CCR7 FITC, CD45 RA APC, CD45RA PE, LAP-1 PerCP-Cy5.5, CD278 (ICOS) PerCP-Cy5.5, anti-CTLA-4 APC with appropriate Isotype control antibodies and Foxp3 Buffer Set were purchased from (BD Biosciences San Jose, CA, USA). CD19 PerCP, CD8 PerCP cy 5.5, CD183 PE, CCR7 FITC, CD45RA APC, CD25 FITC and CD278 (ICOS) Alexa, and anti-CD40/TNFRSF5 were purchased from BD Pharmingen, San Jose, California. EasySep Human CD19 Positive and Human CD8 Positive Selection Kits were purchased from Stem Cell Technologies (Vancouver, British Columbia, Canada). Dynabeads, Human T-Activator CD3/CD28 was purchased from Life technologies (Grand Island, NY, USA). CpG (ODN 2006-G5) was purchased from InvivoGen (San Diego, California).