CD25highCD278+CD183+CD8+ Treg Inhibit
Immunoglobulin Production
Purified B cells were cultured alone or with
CD25highCD278+CD183+CD8+ Treg at B: CD8 Treg ratios of
1:1, 1:1/2, and 1:1/4, and activated with 2ug/ml of anti-human CD40
antibody and 2.5ug/ml of CpG and incubated at 37°C for 14 days. Culture
supernatants were collected and IgM, IgG, and IgA was measured by ELISA
kit against known standards and according manufacturer’s protocol.
Figure 5 show data from 6 normal controls. CD8 Treg inhibited
significantly production of all immunoglobulin isotypes at all ratios,
IgM (<0.0005), IgG (P<0.0039) and IgA (P
<0.0078).