CD25highCD278+CD183+CD8+ Treg inhibit B
cell proliferation
Purified B cells were first labeled with CFSE and then co-cultured
without or with sorted CD25highCD278+CD183+CD8+ Treg
in B: CD8Treg ratio of 1:1, 1:1/2 and 1:1/4 and activated with 2ug/ml of
anti-CD40 and 2.5ug/ml of CpG and cultured for 5 days, and proliferation
was determined by CFSE dilution assay. A representative flow cytometry
histogram is shown in Figure 4A and cumulative data from 6 different
individuals are shown in Figure4B. CD8 Treg, in a
concentration-dependent manner inhibited B cell proliferation at B:CD
8Treg ratio of 1:1 (P< 0.0008), 1:1/2 (P<0.008), and
1:1/4 (P>0.80).