CD25highCD278+CD183+CD8+ Treg Inhibit Immunoglobulin Production
Purified B cells were cultured alone or with CD25highCD278+CD183+CD8+ Treg at B: CD8 Treg ratios of 1:1, 1:1/2, and 1:1/4, and activated with 2ug/ml of anti-human CD40 antibody and 2.5ug/ml of CpG and incubated at 37°C for 14 days. Culture supernatants were collected and IgM, IgG, and IgA was measured by ELISA kit against known standards and according manufacturer’s protocol. Figure 5 show data from 6 normal controls. CD8 Treg inhibited significantly production of all immunoglobulin isotypes at all ratios, IgM (<0.0005), IgG (P<0.0039) and IgA (P <0.0078).