Serological Diagnostic Test
The collected sera were tested by commercially available FMDV-3ABC-Ab
ELISA kit (Non-structural proteins 3 ABC of FMD virus infection) (ID
Screen® FMD NSP Competition, ID-VET, Grabels, France) for the detection
of antibody to 3ABC poly-protein which is a useful indicator of past
FMDV infection regardless of the serotype involved. The 3-ABC-ELISA was
used according to the manufacturer’s instructions. The test principle is
the blocking of plate bound NSP antigen by antibodies present in the
serum samples. Any antibody specific for 3ABC binds to the antigen in
the wells and forms an antigen/antibody complex on the plate well
surface. Antibody to the assay was performed according to manufacturer’s
instruction and results were analyzed and interpreted using: