According to the ELISA test kit manual, the samples were categorized
based on their optical density (OD values as negative if OD value
< 20%, ambiguous if OD value is between 20-30 % positive if
OD value is > 30%).
Data Management and
Analysis
Data generated from laboratory analysis and questionnaire survey were
recorded and coded using Microsoft Excel spreadsheet (Microsoft
Corporation) and analyzed using STATA version 14.0 for Windows (Stata
Corp. College Station, TX, USA). Descriptive statistics (frequency and
percentage) were employed to calculate the proportion of risk factors
for FMD. Individual level animal prevalence was calculated by dividing
the number of animals with positive ELISA tests by the total number of
tested animals, and the herd prevalence was determined by dividing
positive herds by the total number of herds. Herds were considered
positive if one or more animals in the herd had a positive ELISA test.
Risk factors for seroprevalence of FMD virus were investigated using
logistic regression analysis. In all the analyses, confidence levels at
95% were calculated, and a P < 0.05 was used for statistical
significance level.