Results:
The results of this survey showed that 18 (11.6%) bovines and 45
(18.9%) goats were having antibodies specific for CCHFV. Out of 132
CCHF negative bovine samples subjected for PCR amplification targeting
the 312 bp product of cytochrome b gene of T. annulata, 21
samples showed positivity. The overall PCR based prevalence rate of
bovine tropical theileriosis is 15.9 %. Phylogenetic analysis revealed
that Jabalpur T. annulata isolate (GenBank accession no. MN893432
to MN893434) share 100% nucleotide sequences identity with Rewa, Madhya
Pradesh (MG787986.1), Mhow, Madhya Pradesh (MG787985.1), Mathura, Uttar
Pradesh (MG787984.1), Hisar, Haryana (MG787983.1), and Bareilly, Uttar
Pradesh (MG787980.1) isolates (Fig.2). Physical examination of animals
revealed that, animals are severely infested with Hyalomma spp.ticks. Further, farmers covered under survey were scored and analysis
revealed that, they are having lower knowledge on zoonotic diseases and
tick-borne diseases (Table.1). The education level of farmers is
directly correlated with the knowledge of animal husbandry activities.
Interestingly, majority of the farmers reported that, the general
practice of tick control is hand pick the ticks /groom the animal and
crush compared to use of chemical acaricides.