Genomic DNA extraction from blood using a bead-based automated kit
Extraction of genomic DNA from all blood pellet samples with the bead-based automated kit MagMAX™CORE Nucleic Acid Purification Kit (ThermoFisher Scientific, Applied Biosystems, USA) was performed according to the manufacturer’s recommendations. Briefly, 200 µL of sample was mixed with a bead/Proteinase K mix and a lysis/binding solution. The mixture was processed using the KingFisher™ Duo Prime Purification System (ThermoFisher Scientific, USA). Genomic material was eluted in 90 µl of Elution buffer and stored at -20°C until required. The concentration and purity of samples were measured with an IMPLEN Nanophotometer (IMPLEN, Germany).