Figure 4. Off-target analysis in TALEN edited CD4+ T cells. A)
Targeted amplicon sequencing of the top 20 off-targets predicted by
PROGNOS and analyzed by Cripresso2 (** p ≤0,01, *** p≤0,001). B) Oligonucleotide-capture assay (OCA) performed in CD4+ T of
three different donors and measured in triplicate. Bars represent the
mean ±SD of all nine measurements after subtraction of the scores
obtained for mock-treated cells.