Figure 4. Off-target analysis in TALEN edited CD4+ T cells. A) Targeted amplicon sequencing of the top 20 off-targets predicted by PROGNOS and analyzed by Cripresso2 (** p ≤0,01, *** p≤0,001). B) Oligonucleotide-capture assay (OCA) performed in CD4+ T of three different donors and measured in triplicate. Bars represent the mean ±SD of all nine measurements after subtraction of the scores obtained for mock-treated cells.