Figure 3. CCR5 knockout does not impair CD4+ T cell functions. A) Viability. Shown is the viability of CD4+ T cells of several donors 24h after they were either mock-electroporated (Pulse), or electroporated with mRNA (GFP/TALEN L+L/TALEN L+R) relative to untreated (UT) cells (n=4-12; * p ≤0,05; ** p ≤0,01; ***p ≤0,001; ±SD). B-C) Proliferation. Shown are the growth curves of CD4+ T cells subjected to transient cold-shock at 32°C (B) or kept at 37°C (C) after being mock-electroporated (Pulse) or electroporated with mRNA (GFP, TALEN arms L+L/L+R), or left untreated (UT). Cells were counted at the indicated time points. Error bars represent ±SD. D-F) Cytokine secretion. Cytometric bead array was used to assess the release of TNF-α (D), IFN-γ (E) or IL-2 (F) at indicated timepoints after stimulation. Samples were measured in triplicate, error bars represent ±SD.