Figure 2. CCR5 disruption confers HIV-1 resistance. A)
Lentiviral challenge. Untreated (UT) cells as well as TALEN edited cells
(L+L; L+R) were transduced with GFP-expressing lentiviral particles that
were either pseudotyped with VSV-G or HIV-1BaL-gp160. Challenge was
performed at day 7 and day 21 of culture. Indicated is the percentage of
GFP-positive cells relative to the corresponding untreated cells.
Samples derived from two Δ32 heterozygous donor are marked in red. Each
dot represents a different donor (n=3-7; ±SD; ns=not significant, *p ≤0, 05; ** p ≤0,01, paired t -test). B-D)
Challenge with HIV-1. Shown is the p24 kinetics over the course of 12
days of infection in untreated (UT), TALEN edited cells (L+R 100), and
edited cells spiked with UT at ratios of 1:1 (L+R 50) or 1:3 (L+R 25).
Cells were challenged with HIV-1JRFL at an MOI of 0.001
(B), an MOI of 0.01 (C) or with HIV-1NL3-4 at an MOI of
0.01 and 0.001 in triplicates ±SD (D).