Anisidine Value
The measurement of anisidine value carried out according to AOCS Official method Cd, 18-90 (1996). For this purpose, 1g oil sample mixed with iso-Octane into 25ml conical flasks. Then, 5 ml of this solution mixed to 1ml of 0.25% p- anisidine solution in glacial acetic acid. After 10 minutes, solution adsorption read in 350 nm wavelength by spectrophotometer model UV-6600 (spectroFlex, Germany). The anisidine value was calculated from the following equation:
\begin{equation} p-Anisidine\ Value=\frac{25\times(1.2\ As-Ab)}{m}\nonumber \\ \end{equation}
As, Ab and m is illustrative of solution adsorption before and after reaction with p-Anisidine solution, respectively.