Peroxide Value
To determine peroxide value used of A.O.A.C (1984) method. From each of
the oil samples, 2g were weighed into different conical flasks. Then,
15ml of the mixture of CH3COOH and CHCl3in the ratio of 3:2 was added to the oil samples respectively. In the
next step, 0.5ml of saturated potassium iodide was added to each conical
flask and allowed to stand for 5 minutes left in room temperature for 5
minutes, thereafter, 15ml of distilled water was added and titrated with
0.1 N Na 2S2O3 until it appeared yellowish color. Finally, 0.5 ml of
starch indicator was added and the titration continued to a colorless
end-point. The peroxide value was calculated from the equation:
\begin{equation}
\text{PV}=\ \frac{\left(V2-V1\right)\mathsf{\text{\ x}}\text{\ T}}{M}\ \mathsf{x}\ 1000\nonumber \\
\end{equation}where M = mass of oil taken (2g), V₂ = volume of Na₂S₂O₃ , V₁ = volume
of blank and T = normality of Na₂S₂ O₃.