Figure 4. mRNA analysis of CHEK2 c.320-5T>A
Top, schematic representation of CHEK2 c.320-5T>A
splicing effect. cDNA amplification showed a double band, one
corresponding to the full-length transcript (860 bp) and the other to
the transcript lacking exons 3 and 4 (587 bp), as shown in the
electropherogram on the bottom left. Bottom right, agarose gel of a
carrier and 2 controls with (P+) and without puromycin (P-).