2.1 Bacterial strains, plasmids, media, and chemicals
Microbial strains and plasmids used in this work are listed in Table S1. Cells were grown in lysogeny broth (LB) medium[19] or M9* medium [20] with a pH of 7.2 supplemented with 0.5 % (w/v) glucose as sole carbon and energy source. Kanamycin (50 µg mL−1) was applied for selection when necessary. Unless stated otherwise, all chemicals were purchased from Sigma-Aldrich (Steinheim, Germany) or Carl Roth (Karlsruhe, Germany) in the highest purity available and used without further purification. 6HA was acquired from abcr (Karlsruhe, Germany). Molecular biology methods and strain constructions are explained in detail in the Supplementary Information (Sections 1 and 2)