Figure 2: Inhibition studies with P. taiwanensis VLB120 (pSEVA_CHMO). The influence of cyclohexanol (A) or the product ε-CL (B) on specific CHMO activity was investigated in resting cell bioconversions. Cells were cultivated in M9* medium with 0.5 % (w/v) glucose, induced by IPTG for 6h, harvested, and resuspended in KPi-g buffer (100 mM potassium phosphate buffer supplemented with 1 % (w/v) glucose) to a biomass concentration of 0.25 gCDW L-1 in 1 mL liquid volume (A) or 0.5 gCDW L-1 in 10 mL liquid volume (B). Reactions were started by adding 3 mM cyclohexanone. Graphs represent average values and standard deviations of two independent biological replicates.