Isolation of pancreatic mononuclear cells and flow cytometry analysis
Pancreatic mononuclear cells (PMNCs) were isolated from mouse pancreas in accordance with previous reports [23]. PMNCs were stained with FITC or PE-conjugated anti-B220 Ab (eBioscience, San Diego, CA), anti-pDC Ag-1 (PDCA-1) Ab (eBioscience), anti-CD3 Ab (eBioscience), anti-CD11b Ab (eBioscience), or anti-CD11c Ab (eBioscience) and then subjected to flow cytometric analysis using an Accuri C6 flow cytometer (BD Biosciences, San Jose, CA) and CFlow Plus software (BD Biosciences).