Cytokine assays
Cytokine concentrations in the pancreas were measured by the enzyme-linked immunosorbent assay (ELISA), as previously described [24, 25]. Concentrations of IFN-α, IFN-γ, IL-6, IL-13, IL-33, and TNF-α were determined using ELISA kits obtained from R&D Systems (Minneapolis, MN) and eBioscience.