Isolation of pancreatic mononuclear cells and flow cytometry
analysis
Pancreatic mononuclear cells (PMNCs) were isolated from mouse pancreas
in accordance with previous reports [23]. PMNCs were stained with
FITC or PE-conjugated anti-B220 Ab (eBioscience, San Diego, CA),
anti-pDC Ag-1 (PDCA-1) Ab (eBioscience), anti-CD3 Ab (eBioscience),
anti-CD11b Ab (eBioscience), or anti-CD11c Ab (eBioscience) and then
subjected to flow cytometric analysis using an Accuri C6 flow cytometer
(BD Biosciences, San Jose, CA) and CFlow Plus software (BD Biosciences).