Using an acute mouse thrombosis model, where the carotid artery is exposed to ferric-chloride to induce thrombosis, thrombi were successfully visualised via MRI (von zur Muhlen et al., 2008; Ta et al., 2011, 2017), nuclear imaging (Heidt et al., 2011; Ardipradja et al., 2014; Hagemeyer et al., 2014), ultrasound (Wang et al., 2012, 2014, 2016), and optical imaging (Lim et al., 2017). Upon the injection of microparticles, iron oxide (MPIOs) coupled with scFvanti-GPIIb/IIIa, these targeted MPIOs were shown to bind at the site of blood clots, resulting in a decrease of signal intensity in T2-weighted MRI (Figure 9) (von zur Muhlen et al., 2008). The authors further demonstrated that this diminution in signal could be reversed after the injection of urokinase as a fibrinolytic drug, indicating successful thrombolysis (Figure 10). Similarly, scFvanti-GPIIb/IIIa radiolabelled with Indium-111 (111In) and 18F has been used for the imaging of activate platelets in thrombi using SPECT (Figure 11 and Video 1) (Heidt et al., 2011) and PET (Ardipradja et al., 2014), respectively. In another murine study, Ziegler et al. radiolabelled scFvanti-GPIIb/IIIa with 64Cu, and visualised the accumulation of activated platelets in ischemic and then reperfused myocardium (Ziegler et al., 2016). This PET imaging approach showed an uptake of radioactivity in the myocardium of mice, after just a- 10 min period of ischemia (Figure 12).(Ziegler et al., 2016). Noteably, after 10 min there was no increase of classical markers of cardiac ischemia, indicating that the imaging of platelets provides a means of detection of cardiac ischaemia with an unprecedented sensitivity. Using ultrasound, Wang et al. demonstrated direct and immediate visualisation of thrombi using microbubbles coupled with scFvanti-GPIIb/IIIa (Figure 13) (Wang et al., 2012). Realtime molecular ultrasound imaging can be used to image the success or failure of thrombolysis (Figure 14), and importantly, the dislodgment of a thrombus can be directly visualised (Video 2) (Wang et al., 2012). Lim et al. conjugated near-infrared dyes onto scFvanti-GPIIb/IIIa, which allowed early and sensitive detection of thrombi using in vivo three-dimensional optical imaging (Lim et al., 2017).