Reference Barcode Generation
We generated 757 12S MiFish barcodes for 612 California Current fishes, 557 teleosts, 51 elasmobranchs, and 4 cyclostomatan (Supplemental Table 2). Of these, 56 barcodes were duplicates of the same elasmobranch taxa amplified with both the MiFish Elasmobranch and MiFish Universal 12S primer sets. In total, we generated an additional 258 novel 12S barcodes for California Current fishes compared to what was available in GenBank at the time of publication. Combining these barcodes with existing reference sequences deposited on GenBank (October 2019) provides reference 12S barcodes for 715 out of 864 (82.8%) California Current fish species.