CRUX-12S Database vs. Combined Database
Comparing the CRUX-12S and combined database results demonstrated
that the inclusion of novel voucher sequences allowed for species-level
identification for 11 additional California Current taxa including Kelp
bass (Paralabrax clathratus ), California moray (Gymnothorax
mordax ), Opaleye (Girella nigricans ), Giant kelpfish
(Heterostichus rostratus ), Ocean whitefish (Caulolatilus
princeps ), and California halibut (Paralichthys californicus )
(Supplemental Table 3). The use of the FishCARD reference database also
resulted in improved taxonomic classification for 4 species previously
missing barcodes that were assigned to other native California Current
fish species using the combined database (Supplemental Table 3).
Surprisingly, the inclusion of additional California Current-specific
fish 12S barcodes also led to 10 ASVs receiving a less specific
taxonomic classification compared to the CRUX-12S database alone.
Ten ASVs assigned to the California native SeƱorita (Oxyjulis
californica ) by the CRUX-12S database were only identified to
the Family Labridae using FishCARD.