sCSF1R increased the resistance of cells to the chemotherapeutic drug cytarabine
Transcriptome studies have shown that a few monocytes are potential precursors in the circulation for LCH11,12. To evaluate the effect of sCSF1R on the chemosensitivity of LCH cells, we infected THP-1 cells with a BRAF -V600E overexpression lentivirus, which resulted in a significant increase in ERK1/2 phosphorylation (Supplementary Figure S5A). MTS experiments showed significantly elevated IC50 values after incubation with sCSF1R compared to those of the control (IC50: 4.6 vs . 2.3 μmol/L, P = 0.02; Supplementary Figure S5B), indicating increased resistance to cytarabine (Ara-C) resulting from sCSF1R treatment.