Flow cytometry
After surface and intracellular staining, PBMCs were acquired for analysis using an LSRFortessaTM flow cytometer (BD Bioscience), followed by analysis using FlowJo software (Tree Star). At least 500,000 live events were acquired for the lymphocyte and monocyte populations. Fine singlets were constructed from PBMCs by removing doublets and cell clumps (Fig.1a).