Flow cytometry
After surface and intracellular staining, PBMCs were acquired for
analysis using an LSRFortessaTM flow cytometer (BD
Bioscience), followed by analysis using FlowJo software (Tree Star). At
least 500,000 live events were acquired for the lymphocyte and monocyte
populations. Fine singlets were constructed from PBMCs by removing
doublets and cell clumps (Fig.1a).