2.3 Enzyme-linked immunosorbent assay (ELISA) for CN component
specific IgE levels
The αs1-, β-, and κ-CN-specific IgE levels were evaluated by indirect
ELISA.4 The relative immunoglobulin levels (%) for
each sample were calculated based on the reference serum with high
αs1-CN-specific immunoglobulin levels. These measured values were used
in this study.