Figure legends
FIGURE 1 Inhibition assay of casein-specific IgE using ImmunoCAP tests.
Different concentrations of casein (black circles), casein phosphopeptide (white circles), or ovalbumin (gray circle) were mixed with the target serum at a 1:1 ratio and then incubated at 4°C overnight. Casein-specific IgE was measured using ImmunoCAP tests.
FIGURE 2 Basophil activation test stimulated by casein or casein phosphopeptide.
The basophil activation test was performed using an Allergenicity Kit (Beckman Coulter). Different concentrations of casein (black circles) and casein phosphopeptide (white circles) were used as stimulators. The percentage of activated basophils was determined using a threshold CD203c fluorescence intensity based on a comparison between the positive and negative controls.