Figure legends
FIGURE 1 Inhibition assay of casein-specific IgE using
ImmunoCAP tests.
Different concentrations of casein (black circles), casein
phosphopeptide (white circles), or ovalbumin (gray circle) were mixed
with the target serum at a 1:1 ratio and then incubated at 4°C
overnight. Casein-specific IgE was measured using ImmunoCAP tests.
FIGURE 2 Basophil activation test stimulated by casein or
casein phosphopeptide.
The basophil activation test was performed using an Allergenicity Kit
(Beckman Coulter). Different concentrations of casein (black circles)
and casein phosphopeptide (white circles) were used as stimulators. The
percentage of activated basophils was determined using a threshold
CD203c fluorescence intensity based on a comparison between the positive
and negative controls.