Data and Sample Collection
After consent or assent with parental consent was obtained from
survivors and controls, participants filled out a brief history
regarding health history, diet, and exercise. Body weight, height, and
blood pressure were obtained by clinic staff. Clinical lab testing was
performed as indicated for survivors. Serum, plasma, and whole blood
were drawn for later analysis for all subjects at study visit. Stool was
collected at the patient’s home or on-site at the Cleveland Clinic using
an Abbexa (Houston, TX) Feces Catcher stool collection kit, then shipped
on ice to the Genomics Medicine Biorepository within 30 days. The
samples were then aliquoted and centrifuged at 13,200rpm for 15s. The
supernatant was removed, and the fecal pellet was stored at -80°C to
await further processing.