Computational RNA secondary structure prediction and statistical
analysis
Mfold was used for predicting the stability of secondary structures for
CFTR exon 13 WT and all the mutants. Free energy of secondary structures
was calculated using default settings. Statistical analysis was
performed using Microsoft Excel (Microsoft Office Software), GraphPad
Prism (GraphPad Software Inc.) and ImageJ. Statistical analyses were
done by unpaired Student’s T tests (* p < 0.05, ** p
< 0.01 and *** p < 0.001).