Computational RNA secondary structure prediction and statistical analysis
Mfold was used for predicting the stability of secondary structures for CFTR exon 13 WT and all the mutants. Free energy of secondary structures was calculated using default settings. Statistical analysis was performed using Microsoft Excel (Microsoft Office Software), GraphPad Prism (GraphPad Software Inc.) and ImageJ. Statistical analyses were done by unpaired Student’s T tests (* p < 0.05, ** p < 0.01 and *** p < 0.001).