Figure 1. (A) Changes in O-J phase of normalized chlorophyll a
fluorescence intensity in response to 15 min of different light
qualities exposure, respectively. (B) Action spectrum of OEC
photoinhibition. The inset shows the changes in W k following WL
exposure. The means ± SD are calculated from three independent samples.
Figure 2. (A) Changes in normalized chlorophyll a fluorescence intensity
of ΔV t in response to 3 h of BL (blue circles),
WL (light gray triangles) and RL (red inverted triangles) exposure,
respectively. Each curve represents the average of three replicates. (B)
Variations in F v/F m in
response to BL, WL and RL exposure, respectively. (C) Changes in three
peripheral proteins of OEC are analyzed by Western blot. The
significantly different value from dark (Tukey’s tests, p< 0.05) is marked with an asterisk (*). (D) Variations in
OECcenters in response to BL, WL and RL exposure,
respectively. The means ± SD are calculated from three independent
samples. Different letters indicate a significant difference (p< 0.05, one-way ANOVA).
Figure 3. (A) The typical O2 content change curve in the
reaction chamber of the Liquid-Phase Oxygen Electrode System for
monitoring the O2 evolution rate. (B) Time course of
changes in the O2 evolution rate in response to 3 h of
BL, WL and RL exposure, respectively. The means ± SD are calculated from
three independent samples. (C) Changes in the oxygen content change
curve during the O2 evolution rate monitoring after 3 h
of BL, WL and RL exposure, respectively. The rising and falling slope of
the curve under light and darkness represent the net photosynthetic rate
and respiration rate, respectively.
Figure 4. Principal component analysis (PCA) of identified genes. Light
blue squares represent the samples of control, whereas red circles,
green triangles, and dark blue inverted triangles indicate samples
treated by BL, RL, and WL, respectively. Different spot of each color
represents the biological replicates for this treatment.
Figure 5. (A-C) Enriched KEGG pathways among the DEGs in response to the
BL, WL, and RL treatments, respectively. Vertical axis shows the pathway
names, whereas the horizontal axis shows the enrichment factor. The size
of each spot reflects the number of DEGs in this pathway, and each spot
color corresponds to the different P -value ranges. Pathways withP -values < 0.05 are significantly enriched.
Figure 6. (A) Expression profiles of the DEGs related to the carotenoid
biosynthesis pathway. (B) Time course of changes in the carotenoid
contents in response to 3 h of BL, WL and RL exposure. The means ± SD
are calculated from three independent samples. Different letters
indicate a significant difference (p < 0.05, one-way
ANOVA).
Figure 7. (A) Expression profiles of the DEGs related to the
anthocyanins biosynthesis. (B) Time course of changes in the anthocyanin
contents in response to 3 h of BL, WL and RL exposure. The means ± SD
are calculated from three independent samples. Different letters
indicate a significant difference (p < 0.05, one-way
ANOVA).