Figure 1. (A) Changes in O-J phase of normalized chlorophyll a fluorescence intensity in response to 15 min of different light qualities exposure, respectively. (B) Action spectrum of OEC photoinhibition. The inset shows the changes in W k following WL exposure. The means ± SD are calculated from three independent samples.
Figure 2. (A) Changes in normalized chlorophyll a fluorescence intensity of ΔV t in response to 3 h of BL (blue circles), WL (light gray triangles) and RL (red inverted triangles) exposure, respectively. Each curve represents the average of three replicates. (B) Variations in F v/F m in response to BL, WL and RL exposure, respectively. (C) Changes in three peripheral proteins of OEC are analyzed by Western blot. The significantly different value from dark (Tukey’s tests, p< 0.05) is marked with an asterisk (*). (D) Variations in OECcenters in response to BL, WL and RL exposure, respectively. The means ± SD are calculated from three independent samples. Different letters indicate a significant difference (p< 0.05, one-way ANOVA).
Figure 3. (A) The typical O2 content change curve in the reaction chamber of the Liquid-Phase Oxygen Electrode System for monitoring the O2 evolution rate. (B) Time course of changes in the O2 evolution rate in response to 3 h of BL, WL and RL exposure, respectively. The means ± SD are calculated from three independent samples. (C) Changes in the oxygen content change curve during the O2 evolution rate monitoring after 3 h of BL, WL and RL exposure, respectively. The rising and falling slope of the curve under light and darkness represent the net photosynthetic rate and respiration rate, respectively.
Figure 4. Principal component analysis (PCA) of identified genes. Light blue squares represent the samples of control, whereas red circles, green triangles, and dark blue inverted triangles indicate samples treated by BL, RL, and WL, respectively. Different spot of each color represents the biological replicates for this treatment.
Figure 5. (A-C) Enriched KEGG pathways among the DEGs in response to the BL, WL, and RL treatments, respectively. Vertical axis shows the pathway names, whereas the horizontal axis shows the enrichment factor. The size of each spot reflects the number of DEGs in this pathway, and each spot color corresponds to the different P -value ranges. Pathways withP -values < 0.05 are significantly enriched.
Figure 6. (A) Expression profiles of the DEGs related to the carotenoid biosynthesis pathway. (B) Time course of changes in the carotenoid contents in response to 3 h of BL, WL and RL exposure. The means ± SD are calculated from three independent samples. Different letters indicate a significant difference (p < 0.05, one-way ANOVA).
Figure 7. (A) Expression profiles of the DEGs related to the anthocyanins biosynthesis. (B) Time course of changes in the anthocyanin contents in response to 3 h of BL, WL and RL exposure. The means ± SD are calculated from three independent samples. Different letters indicate a significant difference (p < 0.05, one-way ANOVA).