Soluble mediator release by high and low IgE affinity activation
Amongst the 54 mediators detected (table 1), 19 did not change expression in response to IgE activation, among them IL-4, IL-6 and SCF, that were added to the culture medium. 37 mediators were not detected. Among the remaining substances that responded to change in affinity of IgE 24 could be grouped into 3 types of proteins: 10 soluble cytokines and chemokines and 3 soluble cytoplasmic proteins (Figure 2, Table 2), 9 Type I Transmembrane molecules including cytokine receptors and membrane-bound cytokines and 2 Type II Transmembrane molecules (Figure 3, Table 2). A further 6 cytokines and chemokines, 3 transmembrane proteins and 3 cytoplasmic proteins were detected but did not vary significantly (Figure 4). Mediators are presented in order of strength of response in figure 2 and figure 3. In figure 5, momentary distributions of reactants measured at 3, 6 or 24 hours are plotted.