DNA Extraction and Genome Sequencing
DNA was extracted from leaf tissue and sequenced using Nanopore long
reads sequencing technology (Senol et al., 2017). High-quality DNA was
extracted using the Qiagen kit to construct a 1D library, and
single-molecule sequencing of DNA was performed using the GridION X5
(Oxford Nanopore Technology). Following the manufacturer’s data
filtering and quality control, a total of 645,789 reads with an average
read length of 21.1 kb was recovered (13.6 Gb) and, following assembly,
the N50 was 29.9 kb and the longest read was 153.2 kb. Paired-end
Illumina sequencing was for performed for error correction and K-mer
analysis using Illumina’s Genomic DNA Sample Preparation kit and the
Illumina HiSeq X Ten system.