DNA Extraction and Genome Sequencing
DNA was extracted from leaf tissue and sequenced using Nanopore long reads sequencing technology (Senol et al., 2017). High-quality DNA was extracted using the Qiagen kit to construct a 1D library, and single-molecule sequencing of DNA was performed using the GridION X5 (Oxford Nanopore Technology). Following the manufacturer’s data filtering and quality control, a total of 645,789 reads with an average read length of 21.1 kb was recovered (13.6 Gb) and, following assembly, the N50 was 29.9 kb and the longest read was 153.2 kb. Paired-end Illumina sequencing was for performed for error correction and K-mer analysis using Illumina’s Genomic DNA Sample Preparation kit and the Illumina HiSeq X Ten system.