Figure 2. The effects of water temperature and target gene on eDNA decay rate constants. Left, middle, and right graphs show the linear relationships between decay rate constants and temperature targeting all genes (circle), mitochondrial DNA (square), and nuclear DNA (triangle), respectively. Bold and dotted lines indicate the regression line and the corresponding 95% CI estimated by lm and confintfunctions in R, respectively. R² values of the linear regressions are shown in the top-left corner of each figure, and the asterisks indicate the statistical significance of the linear regressions (*, P < 0.05; **, P < 0.01).