FIGURE 2
Figure 2: Proportion of seroreactive animals according to the locations that samples were collected.
From the 20 samples destined to culturing leptospires, only one retrieved viable leptospires in selective medium. The strain was named FN115 and further molecular and serological characterization was performed. Serogrouping of the FN115 strain revealed a strong and specific titration against serogroup Pyrogenes (12,800 for serogroup Pyrogenes, 1,600 for Icterohaemorrhagiae, and 800 for serogroup Canicola). The MLST analysis revealed that the strain FN115 belongs to sequence type 37 of the MLST scheme, which comprises only L. interrogans strains belonging to serogroup Canicola and Pyrogenes, all recovered from dogs and human hosts. All loci presented 100% identity in the BLAST search except sucA, which presented only one mismatch in a homopolymeric region, which are particularly prone to error in Ion Torrent PGM platform.