Maturation conditions and seed collection
One hundred lines of an F7 RIL population obtained fromS. lycopersicum cv. Moneymaker (MM) × S. pimpinellifolium(PI) accession G1.1554 (Voorrips et al. 2000) have been genotyped with 865 single nucleotide polymorphism (SNP) markers. The F8 population was grown in two different nutritional maturation environments as previously described (Geshnizjani et al. 2020). The fully ripened fruits were collected and the seeds were extracted and dried as previously reported (Chapter 2). Finally, the dry seeds were stored in paper bags at 13°C and 30% RH.