Correlation analysis and network construction
R-packages “MASS”, “Hmisc”, “VGAM”, “gplots” and “graphics”
(https://www.r-project.org/) were used for analysis and
construction of the Spearman correlation between all known metabolites
and also between the metabolites and the seed phenotypic traits. In
addition, by using the “rcorr” R-package for each sub-population the
Spearman correlation between the known metabolites was analysed and the
significance level of correlations was described as false discovery rate
(FDR). Correlation values with FDR≤0.05 were used to create a
correlation network for each maternal environment by using Cytoscape
v.3.4.0. The NetworkAnalyser tool of Cytoscape was used to obtain
additional characteristics of the metabolic networks.