Figure 1. Principal component analysis (PCA) of annotated
metabolites in the two parental lines grown in two different nutritional
environments. MM, Solanum lycopersicum (cv. Moneymaker)
shown as triangles; PI, Solanum pimpinellifolium shown
as circles; HP, High phosphate shown in red; LN, Low
nitrate shown in green.
Figure 2. A, Principal component analysis (PCA) of
known metabolites of dry seeds of RILs grown in HP, High
phosphate (green circles) and LN, Low nitrate (red circles)
conditions. B, Loading scores of metabolites for PC1 and PC2.AA, amino acids (red triangles); OA, Organic acids
(yellow triangles); SA, Sugar alcohols (green triangles);Others, Other components (Blue triangle).
Figure 3. Fold change of the metabolites in two nutritional
maternal environments. HP , High phosphate and LN , Low
nitrate. AA , Amino acids; OA , Organic acids;SA , Sugar alcohols.
Figure 4. Metabolite profiles in dry tomato seeds. The
comparison of metabolite content of the 100 tomato RILs grown in two
nutritional maternal environments, HP , High phosphate andLN , Low nitrate.
Figure 5. Correlation networks of known metabolites for each
maternal environment. A , Low nitrate; B , High
phosphate. The colours of the nodes represent the metabolites category.AA , Amino acids; OA , Organic acids; SA , Sugar
alcohols. The Blue and Red colour of the edges (lines)
indicate positive and negative correlations, respectively. The size of
the nodes correlates with the number of connections within the network
(the degree).
Figure 6. Spearman correlation matrix of all pairs of known
metabolites across the whole RIL population derived from S.
lycopersicum cv. Moneymaker and S. pimpinellifolium .
Figure 7. Correlation matrix of metabolites with phenotypic
traits within the whole RIL population derived from S.
lycopersicum cv. Moneymaker and S. pimpinellifolium .
Figure 8. Distribution of metabolite variation within the
genotypes for each nutritional maternal environment with high phosphate(HP) in black and low nitrogen (LN) in grey.
Figure 9. Heatmap of LOD profiles of the mQTLs detected for G
and G×E. A, Heatmap representing the positions of the mQTLs
explained by the genetic component (G); B, Heatmap indicating
the position of the mQTLs affected by genotype by environment
interactions (G×E). The 12 chromosomes of tomato are separated by dashed
lines. Coloured spots indicating the significant QTLs. The blue and
yellow colours show loci where the S. pimpinellifolium and theS.. lycopersicum alleles enhance the metabolite levels,
respectively. Metabolites and their categories are shown at the right
side of the panels.