Figure 1. Principal component analysis (PCA) of annotated metabolites in the two parental lines grown in two different nutritional environments. MM, Solanum lycopersicum (cv. Moneymaker) shown as triangles; PI, Solanum pimpinellifolium shown as circles; HP, High phosphate shown in red; LN, Low nitrate shown in green.
Figure 2. A, Principal component analysis (PCA) of known metabolites of dry seeds of RILs grown in HP, High phosphate (green circles) and LN, Low nitrate (red circles) conditions. B, Loading scores of metabolites for PC1 and PC2.AA, amino acids (red triangles); OA, Organic acids (yellow triangles); SA, Sugar alcohols (green triangles);Others, Other components (Blue triangle).
Figure 3. Fold change of the metabolites in two nutritional maternal environments. HP , High phosphate and LN , Low nitrate. AA , Amino acids; OA , Organic acids;SA , Sugar alcohols.
Figure 4. Metabolite profiles in dry tomato seeds. The comparison of metabolite content of the 100 tomato RILs grown in two nutritional maternal environments, HP , High phosphate andLN , Low nitrate.
Figure 5. Correlation networks of known metabolites for each maternal environment. A , Low nitrate; B , High phosphate. The colours of the nodes represent the metabolites category.AA , Amino acids; OA , Organic acids; SA , Sugar alcohols. The Blue and Red colour of the edges (lines) indicate positive and negative correlations, respectively. The size of the nodes correlates with the number of connections within the network (the degree).
Figure 6. Spearman correlation matrix of all pairs of known metabolites across the whole RIL population derived from S. lycopersicum cv. Moneymaker and S. pimpinellifolium .
Figure 7. Correlation matrix of metabolites with phenotypic traits within the whole RIL population derived from S. lycopersicum cv. Moneymaker and S. pimpinellifolium .
Figure 8. Distribution of metabolite variation within the genotypes for each nutritional maternal environment with high phosphate(HP) in black and low nitrogen (LN) in grey.
Figure 9. Heatmap of LOD profiles of the mQTLs detected for G and G×E. A, Heatmap representing the positions of the mQTLs explained by the genetic component (G); B, Heatmap indicating the position of the mQTLs affected by genotype by environment interactions (G×E). The 12 chromosomes of tomato are separated by dashed lines. Coloured spots indicating the significant QTLs. The blue and yellow colours show loci where the S. pimpinellifolium and theS.. lycopersicum alleles enhance the metabolite levels, respectively. Metabolites and their categories are shown at the right side of the panels.