Sequence analysis Tandem repeat sequences of CVR (B602L)
The analysis of TRS of CVR (Table 4) revealed 8 TRS variants namely:
Tet-10, Tet-13, Tet-18, Tet-20a, Tet-22, Tet-26, Tet-33 and Tet-41 from
samples collected between April 2019 and November 2020. Six of the TRS
variants were new and different from previously reported variants
reported in Nigeria. The TRS variants were recovered as follows: Dawaki
(1), Katsit (6) Numan (1) and Pandam (1). Katsit and Pandam share
similar TRS variants (Tet-13) (Table 4), similarly Tet-13 was recovered
from Katsit and Pandam LPMs.
Figure 3 : A). Katsit live pig market in Zagon Kataf LGA, Kaduna
State Nigeria B). Pandam live pig market in Quan Pan LGA Plateau State,
Nigeria. C: A pig with the classical presentation of African Swine Fever
in a live pig market in Nigeria. D). Moribund pigs are slaughtered
inside a live pig market in Nigeria.
Figure 4: Phylogenetic tree of partial p72 gene nucleotide
sequences of Nigeria ASFV samples collected in live pig markets in
Nigeria The tree was constructed in MEGA X using the Neighbor joining
method with 1,000 bootstrap replicates. The Nigerian ASFV isolates from
this study are highlighted with diamond shape (Red for 2019 and blue for
2020)
Figure 5: Phylogenetic tree of partial p54 gene nucleotide
sequences of Nigeria ASFV samples collected in live pig markets in
Nigeria The tree was constructed in MEGA X using the Minimum-Evolution
method with 1,000 bootstrap replicates. The Nigerian ASFV isolates from
this study are highlighted with diamond shape (Red for 2019 and blue for
2020)
Figure 6: Maximum Likelihood tree based on the partial amino
acid sequence of CD2v protein of the EP424R gene of ASFV samples
collected in live pig markets in Nigeria. The tree was constructed in
MEGA X. The Nigerian ASFV isolates from this study are highlighted with
diamond shape (Red for 2019 and blue for 2020). Bootstrap values greater
than 75% are shown